Clxxxix. Studies on the Metabolism of Pyruvic Acid in Normal and Vitamin B1-deficient States Vi. the Fate of Injected Pyruvate in the Normal Rabbit
نویسندگان
چکیده
THE results of in vitro work have led to the postulation of several possible paths of pyruvate removal in animal tissues; namely (1) the scheme of Toenniessen & Brinkmann [1930] whereby 2 mol. of pyruvic acid are reduced to form 1 mol. of succinic acid, and the latter is then converted,successively into fumaric, malic, oxaloacetic and 1 mol. of pyruvic acid, (2) the scheme of Krebs & Johnson [1937] whereby the oxidation of pyruvate is preceded by an anaerobic change; 2 mol. of pyruvate interact to form 1 mol. each of lactic acid, acetic acid and C02, (3) the oxido-reduction between pyruvate and an aldehyde, such as triosephosphate, giving lactic acid and an acid such as phosphoglyceric acid, (4) a direct oxidation Jike that which exists in the brain [Peters, 1936; 1938; Ochoa & Peters, 1938]. It is also relevant that according to Weil-Malherbe [1937] pyruvic acid is removed more effectively from brain slices in the presence of glucose than in its absence. However, little is known about pyruvic acid metabolism in vivo. The object of the following experiments is to throw some light on these questions. Rabbits were used in most of the experiments. Instead of estimating the changes following administration of pyruvate in the blood, urine or excreta after some time, it was decided to load the rabbit suddenly with a definite amount of pyruvate by intravenous injection lasting for 1-2 min. and study the chemical changes resulting from this immediately after the injection, a few minutes later and in some instances 35 min. later, when the blood pyruvate level had returned to the pre-injection level. Experimental details
منابع مشابه
Clxxxviii. Studies on the Metabolism of Pyruvic Acid in Normal and Vitamin B1-deficient States v. the Effect of Exercise on Blood Pyruvate in Vitamin B1 Deficiency in Man
FOLLOWING the establishment [Platt & Lu, 1939] of the occurrence of increased amounts of pyruvic acid in the body fluids of patients suffering from beri-beri and the studies of Lu [1939] on the effect of muscular activity on the blood pyruvate of the rabbit, it appeared desirable that an investigation should be made of the relationships between pyruvic acid accumulation in the blood, amount of ...
متن کاملXcviii . Studies on the Metabolism of Pyruvic Acid in Normal and Vitamin B 1 - Deficient States
متن کامل
Lxxx. Blood Pyruvate in Vitamin B1 Deficiency
IN the course of recent work on the role of vitamin B, in the metabolism of brain, it was discovered [Peters and Sinclair, 1933] that lactate solutions in which avitaminous pigeon's brain cells had respired for 2 hours gave a positive nitroprusside reaction for pyruvic acid [Simon and Piaux, 1924]. This led to a quantitative investigation of the formation of pyruvic acid during the respiration ...
متن کاملThe Bisulphite-Binding Power of the Blood in Cases of Epidemic Dropsy, Anæmia and Malaria and Its Possible Bearing on a Vitamin-B Deficiency
Peters and Sinclair (1933) have shown that in pigeons suffering from vitamin-B, deficiency the brain, when incubated with lactic acid, produced pyruvic acid in appreciable amount whereas when the brain of normal control birds is substituted this is not produced. Peters and Thompson (1934) further showed that pyruvic acid is probably a normal intermediary in the course of carbohydrate metabolism...
متن کاملThe Bisulphite Binding Power of the Blood in Health and in Disease, with Special Reference to Vitamin B(1) Deficiency.
There is increasing evidence that the biological function of vitamin B1 is concerned with oxidative processes in the living organism. In studies of oxygen utilization by manometric methods, Peters and his coworkers (1, 2) have shown that there is increased oxygen uptake by deficient brain and kidney tissues in vitro following the addition of vitamin B1. In the same laboratory Thompson and Johns...
متن کامل